Pan-Actin Polyclonal Antibody bs-10196R from Bioss Inc.

Product Specs
ItemPan-Actin Polyclonal Antibody
CompanyBioss Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberbs-10196R
Quantity100ul
ApplicationsWB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Concentration1ug/ul
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat, Dog, Rabbit
TargetActin
IsotypeIgG
HostRabbit
Antibody TypePrimary Antibody
NCBI Full Gene Nameactin alpha cardiac muscle 1
NCBI Gene AliasesLVNC4, CMH11, ACTC, CMD1R, ASD5
ClonalityPolyclonal
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
TissueLung, kidney, Kidney
PurityProtein A purified
ImmunogenKLH conjugated peptide, human alpha smooth muscle Actin
Brand/TrademarkBioss

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Bioss Inc.

400 Trade Center, Suite 2990

Woburn, Massachusetts 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

Email: info@biossusa.com
orders@biossusa.com
support@biossusa.com
business@biossusa.com

Company Profile

Website: www.biossusa.com

(1) CERS6-derived ceramides aggravate kidney fibrosis by inhibiting PINK1-mediated mitophagy in diabetic kidney diseaseAmerican journal of physiology. Cell physiologyAugust 1, 2023Wang X, Song M, Li X, Su C, Yang Y, Wang K, Liu C, Zheng Z, Jia Y, Ren S, et al.,Santa Cruz,sc-100554,"1:1,000",https://www.scbt.com/p/lass6-antibody-l-18?requestFrom=search Anti-α-SMA,Bioss,bs-10196R,"1:1,000",http://bioss.com.cn/prolook_03.asp?id=AF08169606017271&pro37=1 Anti-P62,Proteintech,18420-1-AP,"1:1,000",https://www.ptgcn.com/products/SQSTM1-Antibody-18420-1-AP.htm Anti-Parkin,Proteintech,14060-1-AP,"1:1,000",https://www.ptgcn.com/products/PARK2-Antibody-14060-1-AP.htm Anti-PINK1,Proteintech,23274-1-AP,"1:1,000",https://www.ptgcn.com/products/PINK1-Antibody-23274-1-AP.htm Anti-LC3,Proteintech,14600-1-AP,"1:1,000",https://www.ptgcn.com/products/MAP1LC3B-Antibody-14600-1-AP.htm AF488-conjugated

(2) CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescenceInternational journal of biological sciencesJanuary 1, 2023Liu Z, Wu B, Liu X, Wu X, Du J, Xia G, Cai J, Zhu H, Sheng X, Zhang M, et al.materials Antibodies included anti-β-actin (Santa Cruz Biotechnology, sc-47778), anti-α-SMA (Bioss, bs-10196R and Bioss, bsm-33187M), anti-COL1a1 (Bioss, bs-10423R), anti-CD39 (proteintech, 14211-1-AP), anti-CD73

(3) An EPO-loaded multifunctional hydrogel synergizing with adipose-derived stem cells restores neurogenic erectile function via enhancing nerve regeneration and penile rehabilitationBioengineering & translational medicineSeptember 1, 2022Shao J, Nie P, Yang W, Guo R, Ding D, Liang R, Wei B, Wei H.was blocked with 5% skim milk and incubated with primary antibodies including α‐SMA (1:1000, BIOSS, bs‐10196R), eNOS (1:1000, Abcam, ab66127), nNOS (1:1000, Servicebio, GB11145), Caspase‐3 (1:1000, Affinity Biosciences,

(4) miR-335-5p regulates the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection by directly regulating SP1Acta Biochimica et Biophysica SinicaJuly 25, 2022Runwei Ma, Dayong Zhang, Yi Song, Jichang Kong, Chunjie Mu, Pin Shen, Wenting GuiBeyotime) for 2 h at room temperature and then incubated with primary antibodies against α-SMA (1:1000, bs-10196R; Bioss, Beijing, China), SM22α (1:1000, 10493-1-AP; ProteinTech, Rosemont, USA), CNN1 (1:1000, bs-0095R;

(5) Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion FormationOxidative medicine and cellular longevityJanuary 1, 2022Yang L, Li Z, Chen Y, Chen F, Sun H, Zhao M, Chen Y, Wang Y, Li W, Zeng L, et al.cytokeratin 18 (bs-2043R, Bioss, China), Snail (bs-1371R, Bioss, China), α-smooth muscle actin (α-SMA; bs-10196R, Bioss, China), F-actin (FITC-conjugated phalloidin, C1033, Beyotime, China), vinculin (bs-6640R, Bioss,

(6) Inhibition of acylglycerol kinase sensitizes DLBCL to venetoclax via upregulation of FOXO1-mediated BCL-2 expressionTheranosticsJanuary 1, 2022Ning N, Zhang S, Wu Q, Li X, Kuang D, Duan Y, Xia M, Liu H, Weng J, Ba H, et al.against cleaved-caspase3 (Wanlei, WL02117), FOXO1 (CST, #2880), Ki67 (Abcam, ab1667), α-SMA (Bioss, BS-10196R) and BCL-2 (Abclonal, A19693). TUNEL staining was performed with TUNEL label solution (Roche, G1501).

(7) S100A4/TCF Complex Transcription Regulation Drives Epithelial-Mesenchymal Transition in Chronic Sinusitis Through Wnt/GSK-3β/β-Catenin SignalingFrontiers in immunologyJanuary 1, 2022Gong N, Shi L, Bing X, Li H, Hu H, Zhang P, Yang H, Guo N, Du H, Xia M, et al.antibody (Cat. No. 20874-1-AP; 1:500; ProteinTech Group, Rosemont, IL, USA), and α-SMA antibody (Cat. No. bs-10196R; 1:200; Bioss Antibodies, Woburn, MA, USA). Subsequently, the tissue slides were incubated for 1 h with

(8) The Improvement Effect of Sodium Ferulate on the Formation of Pulmonary Fibrosis in Silicosis Mice Through the Neutrophil Alkaline Phosphatase 3 (NALP3)/Transforming Growth Factor-β1 (TGF-β1)/α-Smooth Muscle Actin (α-SMA) PathwayMedical Science MonitorJune 15, 2021Jingyin Han, Yangmin Jia, Shujuan Wang, Xiaoyu Gan254160, Abbiotec, USA), anti-collagen-1 (1: 1000 dilution, AB34710, Abcam), anti-α-SMA (1: 1000 dilution, bs-10196R-2, Bioss, China), anti-p-p38 (1: 1000 dilution, AB195049, Abcam), anti-p38 (1: 1000 dilution, AB170099,

(9) Stimulation of α7 Nicotinic Acetylcholine Receptor by Nicotine Suppresses Decidual M1 Macrophage Polarization Against Inflammation in Lipopolysaccharide-Induced Preeclampsia-Like Mouse ModelFrontiers in ImmunologyApril 28, 2021Xinjia Han, Wei Li, Ping Li, Zheng Zheng, Baohua Lin, Bei Zhou, Kaimin Guo, Ping He, Jinying Yang(rabbit, 1:100, cat#AF5228, Affinity Biosciences, Cincinnati, OH, USA), anti-α-SMA (rabbit, 1:100, cat#bs-10196R, Bioss Biotechnology, Beijing, China); the information for double immunofluorescent staining was as

(10) Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathyAging (Albany NY)March 31, 2021Rui Niu, Ze-Tong Nie, Lin Liu, Yu-Wen Chang, Jian-Qun Shen, Qiong Chen, Li-Jie Dong, Bo-Jie HuBosterbio), CTGF (1:250 dilution, BA0752, Boster), alpha smooth muscle actin (α-SMA, 1:250 dilution, bs-10196R, Bioss), Collagen Type I Alpha 1 (COL1A1, 1:250 dilution, BA0325, Boster), fibronectin (FN, 1:250 dilution,

(11) Baicalin Ameliorates Pancreatic Fibrosis by Inhibiting the Activation of Pancreatic Stellate Cells in Mice with Chronic PancreatitisFrontiers in PharmacologyJanuary 18, 2021Jianwei Fan, Lifang Duan, Nan Wu, Xiaofan Xu, Jiaqi Xin, Shengnan Jiang, Cheng Zhang, Hong Zhang(bs-1101R, Bioss, 1:200), anti-NF-κB p65 antibody (sc-8008, Santa Cruz, 1:200), anti-α-SMA antibody (bs-10196R, Bioss, 1:200), and anti-COL1A1 antibody (sc-25974, Santa Cruz, 1:200). They were then incubated with

(12) Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle CellsFrontiers in cardiovascular medicineJanuary 1, 2021Zheng H, Qiu Z, Chai T, He J, Zhang Y, Wang C, Ye J, Wu X, Li Y, Zhang L, et al.number,Source,Dilutions GluT1,Immunoway,YT1928,Rabbit,"1:1,000" GluT4,Immunoway,YT5523,Rabbit,"1:1,000" SM22,Proteintech,60213-1-lg,Mouse,"1:5,000" α-SMA,Bioss,Bs-10196R,Rabbit,"1:10,000" OPN,BOSTER,BM4208,Rabbit,"1:1,000" MMP2,Proteintech,66366-1-lg,Mouse,"1:5,000" MMP9(anti-human),Abcam,ab76003,Rabbit,"1:2,500" MMP9(anti-mouse),Abcam,ab228402,Rabbit,"1:1,000" β-actin,BOSTER,BM0627,Mouse,"1:10,000" Goat

(13) Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4Cell death & diseaseFebruary 27, 2020Hou L, Le G, Lin Z, Qian G, Gan F, Gu C, Jiang S, Mu J, Ge L, Huang K.with ice PBS and incubated in specific primary antibodies (anti-vimentin (bs-23063R) and anti-α-SMA (bs-10196R), Bioss Inc. Beijing, China). Then cells were washed again and incubated in fluorescein isothiocyanate-labeled

(14) Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF‑β1/Smad/miR‑192 signaling pathwayExperimental and Therapeutic MedicineAugust 14, 2019Qian Mao, Cuicui Chen, Huankun Liang, Shuhai Zhong, Xinbo Cheng, Laiqing Li(1:1,000; cat. no. ab92486, Abcam), Smad3 (1:500; cat. no. ab40845; Abcam) and α-SMA (1:500; cat. no. bs-10196R; BIOSS). After deparaffin (xylene and gradient ethanol) and rehydration, slices were boiled for 15 min

(15) Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with StreptozotocinNatural products and bioprospectingDecember 1, 2018Lu XY, Zhou FH, Dong YQ, Gong LN, Li QY, Tang L, Cai Z, He JY, Liu MH.

(16) Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammationLipids in health and diseaseNovember 6, 2018Zeng Y, Guan M, Li C, Xu L, Zheng Z, Li J, Xue Y.

(17) Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathwayMolecular medicine reportsMarch 1, 2018Park GB, Kim D.1:500; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); and α-smooth muscle actin (α-SMA; cat. no. bs-10196R; 1:500; BIOSS, Beijing, China) Following this, the membranes were incubated with the following secondary

(18) Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migrationMolecular medicine reportsJune 1, 2017Zhou J, Zhang C, Pan J, Chen L, Qi ST.fibroblast markers vimentin (1:500; 550513; BD Biosciences, Inc., Franklin Lakes, NJ, USA) and α-SMA (1:100; bs-10196R; BIOSS, Beijing, China) and negative staining for the epithelial cell marker Pan-CK (dilution, 1:500,

(19) MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2Cell death & diseaseMay 18, 2017Wang Y, Du J, Niu X, Fu N, Wang R, Zhang Y, Zhao S, Sun D, Nan Y.

(20) Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathwayExperimental and therapeutic medicineApril 1, 2017Kim D, Ko HS, Park GB, Hur DY, Kim YS, Yang JW.Taiwan); EBNA1 (no. MA1-7271; 1:50; Thermo Fisher Scientific, Inc.); and α-smooth muscle actin (α-SMA; no. bs-10196R; 1:1,000; Bioss, Inc., Woburn, MA, USA), followed by the following specific secondary antibodies: anti-mouse-horseradish

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Pan-Actin Polyclonal Antibody

Pan-Actin Polyclonal Antibody from Bioss Inc.

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Bioss Inc.

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Pan-Actin Polyclonal Antibody from Bioss Inc.

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